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X-ray crystallography and NMR spectroscopy have established that copper coordinates through a 3N1O equatorial square-planar pyramid configuration: the histidine imidazole nitrogen, the glycine -amino nitrogen, and the deprotonated amide nitrogen of the glycine-histidine peptide bond, with additional oxygen coordination from neighboring complex carboxyl groups

The majority of cells corresponded to M1 macrophages, while only scattered M2 type macrophages were found in control rats In BPC 157 treated rats less than 5 microglial cells were found per 1 mm 2 , primarily M1 macrophages (Figure 14)

Manalo, A